Epigallocatechin-3-gallate inhibits adipogenesis through down-regulation of PPARγ and FAS expression mediated by PI3K-AKT signaling in 3T3-L1 cells

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• 作者：Mengqing Wu^a ; ^b ; ¹ ; Dan Liu^a ; ^b ; ¹ ; Rong Zeng^a ; ^b ; ¹ ; Tao Xian^a ; ^b ; Yi Lu^a ; ^b ; Guohua Zeng^a ; ^b ; Zhangzetian Sun^c ; Bowei Huang^c ; Qiren Huang^a ; ^b ; ^{qrhuang@ncu.edu.cn}
• 关键词：4E-BP1 ; 4E-binding protein1 ; C/EBP ; CCAAT/ enhancer binding proteins ; EGCG ; epigallocatechin-3-gallate ; FAS ; fatty acid synthase ; FOXO1 ; forkhead box-O1 ; HFD ; high fat diet ; IGF ; insulin-like growth factor ; IR ; insulin resistance ; IRS1 ; insulin receptor substrate 1 ; MSCs ; mesenchymal stem cells ; PGC1 ; PPARγ coactivator 1 ; PI3K ; phosphatidylinositol 3 kinase ; PPARγ ; peroxisome proliferator activated receptor γ ; Rb ; retinoblastoma protein ; TG ; triglyceride ; TIF2 ; transcriptional intermediary facto
• 刊名：European Journal of Pharmacology
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：795
• 期：Complete
• 页码：134-142
• 全文大小：1472 K
• 卷排序：795

文摘

Epigallocatechin-3-gallate (EGCG), a major component in green tea, functions as extensive bioactivities including anti-inflammation, anti-oxidation, and anti-cancer. However, little is known about its anti-adipogenesis and underlying mechanisms. The purport of this study sought to investigate effects of EGCG on 3T3-L1 preadipocyte differentiation and to explore its possible mechanisms. The 3T3-L1 cells were induced to differentiate under the condition of pro-adipogenic cocktail with or without indicated EGCG concentrations (10, 50, 100, 200 µM) for 2, 4, 6 and 8 days, respectively. Also, another batch of 3T3-L1 cells was induced under the optimal EGCG concentration (100 µM) with or without SC3036 (PI3K activator, 10 µM) or SC79 (AKT activator, 0.5 µM) for 8 days. Subsequently, the cell viability was examined by MTT assay and the cell morphology was visualized by Oil red O staining. Finally, the mRNA levels including peroxisome proliferator activated receptor γ (PPARγ) and fatty acid synthase (FAS) were detected by quantitative real time PCR, while the protein levels of PPARγ, FAS, phosphatidylinositol 3 kinase (PI3K), insulin receptor substrate1(IRS1), AKT, and p-AKT were measured by immunoblotting analysis. Our results showed that EGCG inhibited adipogenesis of 3T3-L1 preadipocyte in a concentration-dependent manner. Moreover, the inhibitory effects were reversed by SC3036 or SC79, suggesting that the inhibitory effects of EGCG are mediated by PI3K-AKT signaling to down-regulate PPARγ and FAS expression levels. The findings shed light on EGCG anti-adipogenic effects and its underlying mechanism and provide a novel preventive-therapeutic potential for obesity subjects as a compound from Chinese green tea.