Characterization of DNA–Hv1 histone interactions; discrimination of DNA size and shape

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• 作者：George Papadakis ; Achilleas Tsortos ; Konstantinos Mitsakakis ; Electra Gizeli
• 关键词：Acoustic biosensor ; Hv1 histone protein ; DNA shape ; Nucleoprotein complex ; Rigid layer
• 刊名：FEBS Letters
• 出版年：2010
• 出版时间：5 March 2010
• 年：2010
• 卷：584
• 期：5
• 页码：935-940
• 全文大小：622 K

文摘

We have studied the formation of histone Hv1–DNA complexes using an acoustic biosensor and AFM imaging. Our results show that DNA and histone molecules aggregate into amorphous accumulations which form a compact rigid layer on the sensor’s surface. By measuring changes in the acoustic wave amplitude, it was possible to titrate surface bound DNA with Hv1 and discriminate between DNA molecules of different size and shape. From the kinetic analysis of real time data, K_eq was found equal to 3 × 10⁵ M⁻¹.