Mapping Cd2+-induced membrane permeability changes of single live cells by means of scanning electrochemical microscopy
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- 作者：Fraser P. Filice ; Michelle S.M. Li ; Jeffrey D. Henderson ; Zhifeng Ding ; zfding@uwo.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Scanning electrochemical microscopy (SECM) ; COMSOL 3D simulation ; Depth imaging ; Single live cells ; Membrane permeability mapping ; Cd toxicity
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：18 February 2016
- 年：2016
- 卷：908
- 期：Complete
- 页码：85-94
- 全文大小：2098 K
文摘
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Scanning electrochemical microscopy (SECM) was used to map membrane permeability of T24 cells under Cd2+ stress.
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Depth scan SECM imaging generating 2D current maps of live cells relative to electrode position in the x–z or y–z plane.
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Full 3D models were developed for the SECM system of live cells.
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Experimental probe approach curves to any location above the cell membrane are usable.
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The Cd2+ was found to activate cell apoptosis, with cells shrinking in size and the membrane permeability decreasing.