Influence of the ionic strength of acidic background electrolytes on the separation of proteins by capillary electrophoresis
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- 作者：Samya Bekri ; Laurent Leclercq ; Hervé ; Cottet ; herve.cottet@umontpellier.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Capillary coatings ; Polyelectrolyte multilayers ; Protein separation ; Slope plot ; Ionic strength effect ; Electrophoretic mobility modeling
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：5 February 2016
- 年：2016
- 卷：1432
- 期：Complete
- 页码：145-151
- 全文大小：1230 K
文摘
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Electrophoretic behavior of proteins according to the ionic strength in acidic conditions.
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Better selectivity is obtained at low ionic strength.
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Better selectivity on SMIL coatings compared to neutrally coated capillaries.
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Protein electrophoretic mobility decreases by ∼8–15 Tiselius units per ionic strength decade.
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S values for proteins (0.3–0.4) are comparable to moderately charged polyelectrolytes.