Sulfide exposure results in enhanced sqr transcription through upregulating the expression and activation of HSF1 in echiuran worm Urechis unicinctus
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- 作者：Xiaolong Liu ; Zhifeng Zhang ; zzfp107@ouc.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Xiaoyu Ma ; Xueyu Li ; Di Zhou ; Beibei Gao ; Yajiao Bai
- 关键词：SQR ; sulfidequinone oxidoreductase ; HSF1 ; heat shock factor 1 ; DBD ; DNA binding domain ; HR ; heptad repeats domain ; HSE ; heat shock element ; PSU ; practical salinity units ; RACE ; rapid amplification of cDNA ends ; IPTG ; isopropyl β-D-1-thiogalactopyranoside ; ChIP ; chromatin immunoprecipitation ; EMSA ; electrophoretic mobility shift assays ; BSA ; bovine serum albumin ; HRP ; streptavidin&ndash ; horseradish peroxidase ; PAGE ; polyacrylamide gel electrophoresis ; CMV ; cytomegalovirus ; ORF ; open reading frame
- 刊名：Aquatic Toxicology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：170
- 期：Complete
- 页码：229-239
- 全文大小：3435 K
文摘
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Sulfide activates sqr transcription against respiratory toxicity in echiuran cells.
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HSF1 is a key transcription factor to regulate sqr transcription.
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Sulfide could increase expression and activation of HSF1 in time-dependent manner.
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Overexpression of HSF1 could increase activity of sqr proximal promoter.