FleQ of Pseudomonas putida KT2440 is a multimeric cyclic diguanylate binding protein that differentially regulates expression of biofilm matrix components

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• 作者：Marí ; a Antonia Molina-Henares ^{nene.molina@eez.csic.es} ; Marí ; a Isabel Ramos-Gonzá ; lez ^{maribel.ramos@eez.csic.es} ; Abdelali Daddaoua ^{abdelali.daddaoua@eez.csic.es} ; Ana Marí ; a Ferná ; ndez-Escamilla ^{anamfe@eez.csic.es} ; Manuel Espinosa-Urgel ; ^{manuel.espinosa@eez.csic.es}
• 关键词：Biofilm regulation ; Exopolysaccharide ; Isothermal titration microcalorimetry ; Electrophoretic mobility shift assay
• 刊名：Research in Microbiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：168
• 期：1
• 页码：36-45
• 全文大小：1435 K
• 卷排序：168

文摘

The intracellular signal molecule cyclic di-GMP (c-di-GMP) is an important element in regulation of biofilm formation by bacteria. In Pseudomonas aeruginosa, FleQ functions as a c-di-GMP-dependent transcriptional regulator of expression of flagellar genes and the exopolysaccharide (EPS) Pel, a component of the biofilm extracellular matrix. In the plant-beneficial bacterium Pseudomonas putida KT2440, a mutation in fleQ reduces biofilm formation and colonization of plant surfaces. Using isothermal titration calorimetry and electrophoretic mobility shift assays, we show in this work that FleQ of P. putida interacts with c-di-GMP and directly binds the promoter regions of flagellar and EPS genes. Data obtained by analytical gel filtration and ultracentrifugation indicate that FleQ is in multiple oligomeric states in solution (dimers, tetramers and hexamers), which do not show altered equilibrium in the presence of c-di-GMP. DNA binding is independent of c-diGMP, although it is favored by the second messenger in the case of the promoter of the operon responsible for synthesis of the species-specific EPS Pea. Analysis of expression using transcriptional fusions showed an influence of FleQ upon two of the four EPS operons under regular growth conditions. Finally, a consensus sequence for promoter recognition by FleQ in P. putida is also proposed.