Leishmania infantum mimotopes and a phage-ELISA assay as tools for a sensitive and specific serodiagnosis of human visceral leishmaniasis

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• 作者：Beatriz C.S. Salles^a ; ¹ ; Lourena E. Costa^a ; ¹ ; Patrí ; cia T. Alves^b ; Ana C.S. Dias^b ; Emí ; lia R. Vaz^b ; Daniel Menezes-Souza^a ; ^c ; Fernanda F. Ramos^a ; Mariana C. Duarte^a ; ^c ; Bruno M. Roatt^c ; Miguel A. Chá ; vez-Fumagalli^a ; Carlos A.P. Tavares^d ; Denise U. Gonç ; alves^a ; Regina L. Rocha^a ; Luiz R. Goulart^b ; ^e ; ² ; ^{lrgoulart@ufu.br} ; Eduardo A.F. Coelho^a ; ^c ; ² ; ^{eduardoferrazcoelho@yahoo.com.br}
• 关键词：Visceral leishmaniasis ; Serodiagnosis ; Mimotopes ; Phage display ; Phage&ndash ; ELISA ; Specificity
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：87
• 期：3
• 页码：219-225
• 全文大小：439 K
• 卷排序：87

文摘

A subtractive phage display selection was performed. Eight Leishmania infantum specific mimotopes were selected. Phage clones expressing these mimotopes were amplified. Phages were evaluated for the serodiagnosis of human visceral leishmaniasis. They showed high sensitivity and specificity values in a phage-ELISA assay.