Three-dimensional visualization of membrane phospholipid distributions in Arabidopsis thaliana seeds: A spatial perspective of molecular heterogeneity

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• 作者：Drew Sturtevant^a ; ¹ ; ^{drewsturtevant@my.unt.edu} ; Maria Emilia Dueñ ; as^b ; ^c ; ² ; ^{mduenas@iastate.edu} ; Young-Jin Lee^b ; ^c ; ² ; ^{yjlee@iastate.edu} ; Kent D. Chapman^a ; ¹ ; ^{chapman@unt.edu}
• 关键词：A. thaliana ; Arabidopsis thaliana ; C. sativa ; Camelina sativa ; G. hirsutum ; Gossypium hirsutum ; B. napus ; Brassica napus ; TAG(s) ; Triacylglycerol(s) ; PC ; Phosphatidylcholine ; PI ; Phosphatidylinositol ; MALDI-MSI ; Matrix assisted laser desorption/ionization-mass spectrometry imaging ; ESI-MS ; Electrospray ionization-mass spectrometry
• 刊名：Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：1862
• 期：2
• 页码：268-281
• 全文大小：3148 K
• 卷排序：1862

文摘

3D phospholipid distributions in arabidopsis seeds reveal marked heterogeneity. Quantification of PC and PI species in seed tissues from virtual dissected MS images Acyl lipid mutants showed altered patterns of PC and PI distributions in seed tissues. Distributions visualized by MALDI-MSI in situ were validated by ESI-MS in extracts