Efficient display of scFv antibodies on bacteriophage lambda
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- 作者：Paola Vaccaro ; Emiliano Pavoni ; Giorgia Monteriù ; Pucci Andrea ; Franco Felici and Olga Minenkova
- 关键词：Lambda display ; gpD ; Bacteriophage lambda assembly ; Recombinant scFv antibody
- 刊名：Journal of Immunological Methods
- 出版年：2006
- 出版时间：20 March 2006
- 年：2006
- 卷：310
- 期：1-2
- 页码：149-158
- 全文大小：357 K
文摘
In the present work we demonstrate the efficient display of functional scFv antibodies on the bacteriophage lambda capsid. A single-chain (scFv) anti-CEA antibody gene was cloned in two different vectors to obtain fusion of the scFv antibody to the N- or C-terminus of the bacteriophage lambda capsid protein D (gpD). Lambda bacteriophage assembly occurs in the reducing environment of the cytoplasm; despite this the lambda-displayed anti-CEA antibody fragments retain the capacity to recognize the antigen, indicating correct single-chain antibody folding.
Efficient production of functional scFv exposed on lambda capsid with viable antigen binding specificity allowed us to study and compare the capacity of display, the stability of recombinant antibody expression and the assembly efficiency of bacteriophage particles decorated with recombinant antibody fused to the amino- or carboxy-terminus of lambda D protein.