Enantiomeric separation of pharmaceutically important drug intermediates using a Metagenomic lipase and optimization of its large scale production

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• 作者：Rakesh Kumar^a ; ¹ ; Linga Banoth^b ; Uttam Chand Banerjee^b ; Jagdeep Kaur^a ; ^{jagsekhon@yahoo.com}
• 关键词：Lipase ; transesterification ; Ionic liquid ; kinetic resolution ; Naphthyl ethanol ; Indanol ; Fermentation ; agitation ; aeration
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：995-1003
• 全文大小：1757 K
• 卷排序：95

文摘

In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity = 87m6 U/mg) showed maximum conversion in presence of ionic liquid with Naphthyl-ethanol (eeP = 99%), Indanol and Methyl-4 pyridine methanol (eeS of 98% and 99%) respectively in 1 h. Vinyl acetate was found as suitable acyl donor in transesterification reactions. It was interesting to observe that maximum eeP of 85% was observed in just 15 min with 1-indanol. As this enzyme demonstrated pharmaceutical applications, attempts were made to scale up the enzyme production on a pilot scale in a 5 litre bioreactor. Different physical parameters affecting enzyme production and biomass concentration such as agitation rate, aeration rate and inoculum concentration were evaluated. Maximum lipase activity of 8463 U/ml was obtained at 7 h of cultivation at 1 lpm, 300 rpm and 1.5% inoculum.