One facile fluorescence strategy for sensitive detection of endonuclease activity using DNA-templated copper nanoclusters as signal indicators
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- 作者：Hengzhi ZhaoAuthor Vitae ; Jingjing DongAuthor Vitae ; Fulin ZhouAuthor Vitae ; Baoxin Li ; libaoxin@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; libaoxin@snnu.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Endonuclease activity ; Copper nanoclusters ; Fluorescence assay ; Label-free
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：238
- 期：Complete
- 页码：828-833
- 全文大小：1141 K
文摘
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A simple and facile method is proposed for sensitive detection of endonuclease activity.
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10">DNA-templated CuNCs can be used as signal indicators for fluorescence assay of endonuclease activity.
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15">The method offers a convenient “mix-and-detect” homogeneous assay format without separation, precipitation and washing.
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The whole assay process can be completed in a single tube.
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