Characteristics of Genome Editing Mutations in Cereal Crops

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• 作者：Changfu Zhu¹ ; ⁶ ; Luisa Bortesi² ; ⁶ ; Can Baysal¹ ; Richard M. Twyman³ ; Rainer Fischer² ; ⁴ ; Teresa Capell¹ ; Stefan Schillberg⁴ ; Paul Christou¹ ; ⁵ ; ^{christou@pvcf.udl.cat}
• 关键词：clustered regularly interspaced short palindromic repeats (CRISPRs) ; designer nuclease ; meganuclease ; plant genome editing ; transcription activator-like effector nucleases (TALENs) ; targeted mutation ; zinc-finger endonucleases (ZFNs)
• 刊名：Trends in Plant Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：22
• 期：1
• 页码：38-52
• 全文大小：2623 K
• 卷排序：22

文摘

Designer nucleases allow the creation of new plant genotypes by introducing precisely-targeted double-strand breaks that are resolved by endogenous repair pathways. The major nuclease technologies are meganucleases, zinc-finger nucleases, transcription activator-like effector nucleases, and the CRISPR/Cas9 system. Each comprises a promiscuous endonuclease guided by protein–DNA or RNA–DNA interactions. A great deal is known about the principles of designer nucleases but much remains to be learned about their detailed behavioral characteristics in different plant species. The outcome of genome engineering reflects the intrinsic properties of each nuclease and target genome, causing variations in efficiency, accuracy, and mutation structure. In this article, we critically discuss the activities of designer nucleases in different cereals representing a broad range of genome characteristics.