Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities

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• 作者：Liang Liu¹ ; Xueyan Li¹ ; ² ; Jiuyu Wang¹ ; Min Wang¹ ; Peng Chen¹ ; ² ; Maolu Yin¹ ; ² ; Jiazhi Li¹ ; ² ; Gang Sheng¹ ; Yanli Wang¹ ; ² ; ³ ; ⁴ ; ^{ylwang@ibp.ac.cn}
• 关键词：CRISPR-Cas ; C2c2 ; RNA-guided RNA cleavage ; precursor crRNA ; endonuclease ; HEPN domain
• 刊名：Cell
• 出版年：2017
• 出版时间：12 January 2017
• 年：2017
• 卷：168
• 期：1-2
• 页码：121-134.e12
• 全文大小：12828 K
• 卷排序：168

文摘

Crystal structure of Leptotrichia shahii C2c2-crRNA binary complex and apo form A bulge-containing crRNA stem is essential for C2c2 RNase activities Helical-1 domain has the catalytic site for pre-crRNA processing Promiscuous RNA cleavage due to distance between HEPN catalytic site and crRNA guide