The endothelial nitric oxide synthase (eNOS) polymorphism in otitis media with effusion (OME)

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• 作者：M. Ates^a ; C. Cevik^a ; R. Dokuyucu^b ; ^c ; ^{drecepfatih@gmail.com} ; O. Berber^a ; S. Colak^a ; M. Izmirli^d
• 关键词：Otitis media with effusion ; eNOS polymorphism ; Serous otitis ; Turkey
• 刊名：European Annals of Otorhinolaryngology, Head and Neck Diseases
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：134
• 期：1
• 页码：3-5
• 全文大小：475 K
• 卷排序：134

文摘

Otitis media with effusion (OME) is the most common disease after viral infections of upper respiratory tract (URTI) in children. Studies indicate the important role of nitric oxide (NO) in the etiology of hearing loss. However, there is no study that focuses on the role of nitric oxide synthase (eNOS) polymorphisms in the cases with OME. The aim of the present study is to evaluate the eNOS polymorphisms in the pediatric patients with OME.Materials and methodsEighty-nine patients who are diagnosed with otitis media with effusion and 85 healthy subjects who are compatible in terms of age and gender were included in the study. All patients in the study were subjected to complete ear, nose, throat (ENT) and audiological examinations. DNA analysis was performed with polymerase chain reaction (PCR) technique from the blood samples. The PCR product was cut by restriction fragment length polymorphism (RFLP) with BanII enzyme and checked by agarose gel electrophoresis.ResultsAs a result of genetic analysis, there is no significant difference between patients and the controls in terms of eNOS Glu298Asp polymorphism (G/G, G/T, T/T). When these groups were compared in terms of allele distributions, a significant relationship was found between the patients and the controls (P = 0.037).ConclusionTo the best of our knowledge, G allele was identified as predisposing to the development of OME and this is the first report indicates the correlation between the eNOS G894T polymorphism and OME in Turkey.