Active site structure of SoxB-type cytochrome bo₃ oxidase from thermophilic Bacillus

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• 作者：Uchida ; Takeshi ; Tsubaki ; Motonari ; Kurokawa ; Tatsuki ; Hori ; Hiroshi ; Sakamoto ; Junshi ; Kitagawa ; Teizo ; et. al.
• 关键词：SoxB-type heme-copper oxidase ; Cytochrome bo₃ ; Respiratory chain ; EPR spectroscopy ; Resonance Raman spectroscopy ; EPR ; electron paramagnetic resonance ; RR ; resonance Raman
• 刊名：Journal of Inorganic Biochemistry
• 出版年：2000
• 出版时间：November, 2000
• 年：2000
• 卷：82
• 期：1-4
• 页码：65-72
• 全文大小：224 K

文摘

Two-subunit SoxB-type cytochrome c oxidase in Bacillus stearothermophilus was over-produced, purified, and examined for its active site structures by electron paramagnetic resonance (EPR) and resonance Raman (RR) spectroscopies. This is cytochrome bo₃ oxidase containing heme B at the low-spin heme site and heme O at the high-spin heme site of the binuclear center. EPR spectra of the enzyme in the oxidized form indicated that structures of the high-spin heme O and the low-spin heme B were similar to those of SoxM-type oxidases based on the signals at g