LC-MS analysis, anticancer, antioxidant and antimalarial activities of Cynodon dactylon L. extracts

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• 作者：Daycem Khlifi^a ; ^b ; El Akrem Hayouni^c ; Alexis Valentin^d ; Sylvie Cazaux^a ; Bé ; atrice Moukarzel^d ; Moktar Hamdi^b ; Jalloul Bouajila^a ; ^{jalloul.bouajila@univ-tlse3.fr}
• 关键词：Cynodon dactylon ; LC&ndash ; MS ; Anthocyanins ; Antioxidant activity ; Antimalarial activity ; Anticancer activity
• 刊名：Industrial Crops and Products
• 出版年：2013
• 出版时间：February, 2013
• 年：2013
• 卷：45
• 期：Complete
• 页码：240-247
• 全文大小：626 K

文摘

Extracts (petroleum ether, dichloromethane, acetone, methanol/water (3/1) and water) from Cynodon dactylon L. were obtained by sequential method. The chemical composition of phenolics (6.9-57.41 g gallic acid equivalents/kg of dry mass), tannins (2.49-12.32 g catechin equivalent/kg of dry mass), anthocyanins (0-5.98 g cyanidin equivalent/kg of dry mass) and flavonoids (0-5.02 g quercetin equivalent/kg of dry mass) was evaluated. The extracts were evaluated for their antioxidant (DPPH and ABTS assays), antimalarial (Plasmodium falciparum) and anticancer (MCF-7) activities; the strongest activities were obtained by the water extract (IC₅₀ = 57.21 ¡À 1.47 mg/L), the acetonic extract (IC₅₀ = 38 mg/L) and the petroleum ether extract (IC₅₀ = 39 mg/L), respectively. The results indicate a good correlations between anthocyanins quantity and the antimalarial activity (R² = 0.79) and also to anticancer activity (R² = 0.78). The LC-MS analysis of the extracts having the good activities anticancer and antimalarial, has revealed the presence of seven anthocyanins (delphinidin-3-O-acetylglucoside, petunidin-3-O-caffeoylglucoside-5-O-glucoside, petunidin-3-O-coumarylglucoside-5-O-glucoside, malvidin-3-O-monoglucoside, delphinidin-3-O-acetylglucoside-pyruvic acid, petunidin-3-O-acetylglucoside-5-O-glucoside and cyanidin-3,5-O-diglucoside), identified for the first time in this plant.