Point mutation Gln121-Arg increased temperature optima of Bacillus lipase (1.4 subfamily) by fifteen degrees
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- 作者：Shelly Goombera ; shelly.sahni@rediffmail.com" class="auth_mail" title="E-mail the corresponding author ; Rakesh Kumarc ; Ranvir Singhb ; Neelima Mishrad ; Jagdeep Kaura
- 关键词：Directed evolution ; Lipase ; Error prone PCR ; Thermostability ; Homolog modelling
- 刊名：International Journal of Biological Macromolecules
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：88
- 期：Complete
- 页码：507-514
- 全文大小：1887 K
文摘
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Bacillus lipase LipJ of 1.4 subfamily was randomly mutated by error prone PCR.
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Variants obtained were screened and selected for enhanced thermostability.
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Point mutant Gln121Arg demonstrating increased thermostability was characterized.
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Homolog modelling predicted anchoring of surface loop as molecular basis of thermostability.