Estimation of flux distribution in metabolic networks accounting for thermodynamic constraints: The effect of equilibrium vs. blocked reactions
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- 作者：L. Angeles-Martinez ; C. Theodoropoulos ; k.theodoropoulos@manchester.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：13dpg ; 3-phospho-d-glyceroyl phosphate ; 2pg ; glycerate 2-phosphate ; 3pg ; 3-phospho-d-glycerate ; 6pgc ; 6-phospho-d-gluconate ; 6pgl ; 6-phospho-d-glucono-1 ; 5-lactone ; ac ; acetate ; acald ; acetaldehyde ; accoa ; acetyl-CoA ; actp ; acetyl phosphate ; coa ; coenzyme A ; dhap ; dihydroxyacetone phosphate ; e4p ; erythrose 4-phosphate ; etoh ; ethanol ; f6p ; fructose-6-phosphate ; fdp ; fructose 1 ; 6-bisphosphate ; for ; formate ; fum ; fumarate ; g3p ; glyceraldehyde 3-phosphate ; g6p ; glucose 6-phosphate ; glyc ; glycerol ; gl
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：105
- 期：part_PB
- 页码：347-357
- 全文大小：1952 K
文摘
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We develop a new thermodynamically-constrained metabolic flux analysis method.
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We compute thermodynamically feasible flux ranges for A. succinogenes metabolism.
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A narrower range of fluxes is predicted for the equilibrium case than the block one.
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We systematically evaluate effects of changes in pH, ionic strength and temperature.
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Thermodynamic data uncertainty has dominant contribution in estimating flux ranges.