Production of d-ribose by metabolically engineered Escherichia coli

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• 作者：Hae-Chul Park^a ; ¹ ; Yun-Jung Kim^b ; ¹ ; Chang-Wan Lee^b ; Yong-Taek Rho^b ; JeongWoo Kang^a ; Dae-Hee Lee^c ; Yeong-Je Seong^d ; Yong-Cheol Park^d ; Daesang Lee^e ; Sung-Gun Kim^b ; ^{sgkim@yd.ac.kr}
• 关键词：d-Ribose ; Xylose ; Escherichia coli ; Transketolase ; Catabolite repression
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：52
• 期：Complete
• 页码：73-77
• 全文大小：950 K
• 卷排序：52

文摘

Transketolase-deficient E. coli was constructed to produce d-ribose from xylose. d-Ribose produced in the E. coli was identical to standard d-ribose in HPLC and LC/MS analyses. The transketolase-deficient E. coli was further engineered to uptake glucose and xylose simultaneously for facilitating d-ribose production. The simultaneous uptake of glucose and xylose in the E. coli resulted in a 5-fold improvement of d-ribose production.