Inula japonica extract inhibits mast cell-mediated allergic reaction and mast cell activation

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• 作者：Yue Lu ; Ying Li ; Meihua Jin ; Ju Hye Yang ; Xian Li ; Guang Hsuan Chao ; Hyo-Hyun Park ; Young Na Park ; Jong Keun Son ; Eunkyung Lee ; Hyeun Wook Chang
• 关键词：IFE ; Inulae Flos extract ; BMMCs ; bone marrow-derived mast cells ; IL-3 ; interleukin-3 ; LTC4 ; leukotriene C4 ; SCF ; stem cell factor ; cPLA2 ; cytosolic phospholipase A2 ; MAPKs ; mitogen-activated protein kinases ; PLC¦Ã ; phospholipase C¦Ã ; AA ; arachidonic acid
• 刊名：Journal of Ethnopharmacology
• 出版年：2012
• 出版时间：30 August, 2012
• 年：2012
• 卷：143
• 期：1
• 页码：151-157
• 全文大小：550 K

文摘

Ethnopharmacological relevance

The flowers of Inula japonica (Inulae Flos) have long been used in traditional medicine for the treatment of bronchitis, digestive disorders, and inflammation. However, the mechanisms underlying its anti-inflammatory effects remain yet to be elucidated. The objectives of this study were 1) to assess the anti-allergic activity of the ethanol extract of flowers of Inula japonica extract (IFE) in vivo, 2) to investigate the mechanism of its action on mast cells in vitro, and 3) to identify its major phytochemical compositions.

Materials and methods

The anti-allergic activity of IFE was evaluated using mouse bone marrow-derived mast cells (BMMCs) in vitro and a passive cutaneous anaphylaxis (PCA) animal model in vivo. The effects of IFE on mast cell activation were evaluated in terms of degranulation, eicosanoid generation, Ca²⁺ influx, and immunoblotting of various signaling molecules.

Results

IFE inhibited degranulation and the generation of eicosanoids (PGD₂ and LTC₄) in stem cell factor (SCF)-stimulated BMMCs. Biochemical analysis of the SCF-mediated signaling pathways demonstrated that IFE inhibited the activation of multiple downstream signaling processes including mobilization of intracellular Ca²⁺ and phosphorylation of the mitogen-activated protein kinases (MAPKs), PLC¦Ã1, and cPLA₂ pathways. When administered orally, IFE attenuated the mast cell-mediated PCA reaction in IgE-sensitized mice. Its major phytochemical composition included three sesquiterpenes, 1-O-acetylbritannilactone, britanin and tomentosin.

Conclusions

This study suggests that IFE modulates eicosanoids generation and degranulation through the suppression of SCF-mediated signaling pathways that would be beneficial for the prevention of allergic inflammatory diseases. Anti-allergic activity of IFE may be in part attributed particularly to the presence of britanin and tomentosin as major components evidenced by a HPLC analysis.