Characterization of charge variants of a monoclonal antibody using weak anion exchange chromatography at subunit levels

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• 作者：Gomathinayagam Ponniah ; Christine Nowak ; Alyssa Neill ; Hongcheng Liu ; ^{LiuH@alxn.com}
• 关键词：Recombinant monoclonal antibodies ; LC-MS ; Posttranslational modifications ; Succinimide
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：520
• 期：Complete
• 页码：49-57
• 全文大小：1774 K
• 卷排序：520

文摘

An antibody was analyzed using weak cation exchange chromatography at intact and subunit level after IdeS digestion. Modifications were identified by LC-MS analysis of intact and subunit molecular weights, and peptide mapping. The antibody with an succinimide from aspartate isomerization was identified as an acidic species.