Effects of branched-chain amino acids supplementation on both plasma amino acids concentration and muscle energetics changes resulting from muscle damage: A randomized placebo controlled trial

详细信息    查看全文

• 作者：Alexandre Fouré ; ^a ; ^{alexandre.foure@univ-amu.fr" class="auth_mail" title="E-mail the corresponding author} ; ^{alexandre.foure@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; Kazunori Nosaka^b ; Marguerite Gastaldi^c ; Jean-Pierre Mattei^a ; ^d ; Hé ; lè ; ne Boudinet^e ; Maxime Guye^a ; ^e ; Christophe Vilmen^a ; Yann Le Fur^a ; David Bendahan^a ; Julien Gondin^a
• 关键词：Double blind randomized placebo controlled trial ; Exercise induced-muscle damage ; Magnetic resonance spectroscopy
• 刊名：Clinical Nutrition
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：35
• 期：1
• 页码：83-94
• 全文大小：1551 K

文摘

Branched-chain amino acids promote muscle-protein synthesis, reduce protein oxidation and have positive effects on mitochondrial biogenesis and reactive oxygen species scavenging. The purpose of the study was to determine the potential benefits of branched-chain amino acids supplementation on changes in force capacities, plasma amino acids concentration and muscle metabolic alterations after exercise-induced muscle damage.

Methods

³¹P magnetic resonance spectroscopy and biochemical analyses were used to follow the changes after such damage. Twenty six young healthy men were randomly assigned to supplemented branched-chain amino acids or placebo group. Knee extensors maximal voluntary isometric force was assessed before and on four days following exercise-induced muscle damage. Concentrations in phosphocreatine [PCr], inorganic phosphate [Pi] and pH were measured during a standardized rest-exercise-recovery protocol before, two (D2) and four (D4) days after exercise-induced muscle damage.

Results

No significant difference between groups was found for changes in maximal voluntary isometric force (−24% at D2 and −21% at D4). Plasma alanine concentration significantly increased immediately after exercise-induced muscle damage (+25%) in both groups while concentrations in glycine, histidine, phenylalanine and tyrosine decreased. No difference between groups was found in the increased resting [Pi] (+42% at D2 and +34% at D4), decreased resting pH (−0.04 at D2 and −0.03 at D4) and the slower PCr recovery rate (−18% at D2 and −24% at D4).

Conclusions

The damaged muscle was not able to get benefits out of the increased plasma branched-chain amino acids availability to attenuate changes in indirect markers of muscle damage and muscle metabolic alterations following exercise-induced muscle damage.