Sensitive detection of adenosine triphosphate by exonuclease III-assisted cyclic amplification coupled with surface plasmon resonance enhanced fluorescence based on nanopore

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• 作者：Xuemei Li^aAuthor Vitae ; Yan Wang^bAuthor Vitae ; Jie Luo^bAuthor Vitae ; Shiyun Ai^c ; ^{Ashy@sdau.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Cyclic amplification ; Fluorescence enhancement ; Adenosine triphosphate ; Exonuclease exonuclease III ; Nanopore
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：2 June 2016
• 年：2016
• 卷：228
• 期：Complete
• 页码：509-514
• 全文大小：1408 K

文摘

In traditional bioanalysis with mesoporous silica nanoparticle (MSN) as carrier, fluorescence quenching by encapsulation of fluorescence molecules in the pores was generally used. In the present work, Au nanoparticle@SiO₂ mesoporous silica core–shell nanoparticles (AuNP@SiO₂ MSN) was synthesized, and surface plasmon resonance enhanced fluorescence localized on nanopore was observed. In-hole fluorescence enhancement by MSN for detection of adenosine 5′-triphosphate (ATP) was developed, on the base of exonuclease III (Exo III)-assisted cyclic amplification and surface plasmon resonance enhanced fluorescence localized on nanopore. Because of the autocatalytic target recycling amplification and the fluorescence enhancement, this designed protocol provided an ultrasensitive detection of ATP down to 0.1 nM level, and can be utilized into cell lysates analysis.