Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding
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- 作者：Da-Chuan Piaoa ; Yoon-Seok Leeb ; Jin-Duck Bokb ; Chong-Su Choa ; Zhong-Shan Hongc ; Sang-Kee Kangb ; kangsk01@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Yun-Jaie Choia ; c ; cyjcow@snu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：PEDV S protein ; Inclusion bodies ; Solubilization ; Refolding ; Immunogenicity ; Subunit vaccine
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：77-83
- 全文大小：883 K
文摘
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Expression of truncated S25-229 of PEDV variant strain in Escherichia coli BL21 (DE3) resulted in inclusion body formation.
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Soluble S25-229 was recovered by solubilization and refolding, followed by one-step cation exchange chromatography.
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The CD spectrum of S25-229 showed the characteristic pattern of alpha helical structure.
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The S25-229 showed immunogenicity and elicited strong humoral immune response in mice.
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