Liquid chromatography-tandem mass spectrometric assay for the tyrosine kinase inhibitor afatinib in mouse plasma using salting-out liquid-liquid extraction
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- 作者：Rolf W. Sparidansa ; R.W.Sparidans@uu.nl" class="auth_mail" title="E-mail the corresponding author ; Stephanie van Hoppeb ; s.v.hoppe@nki.nl" class="auth_mail" title="E-mail the corresponding author ; Johannes J.M. Rooda ; J.J.M.Rood@uu.nl" class="auth_mail" title="E-mail the corresponding author ; Alfred H. Schinkelb ; a.schinkel@nki.nl" class="auth_mail" title="E-mail the corresponding author ; Jan H.M. Schellensa ; c ; j.schellens@nki.nl" class="auth_mail" title="E-mail the corresponding author ; Jos H. Beijnena ; c ; d ; jos.beijnen@slz.nl" class="auth_mail" title="E-mail the corresponding author
- 关键词：AUC ; area under the plasma concentration&ndash ; time curve ; CHMP ; Committee for Medicinal Products for Human Use ; EGFR ; epidermal growth factor receptor ; FDA ; Food and Drug Administration ; FVB ; Friend Leukemia Virus ; HER ; human epidermal growth-factor receptor ; NSCLC ; non-small cell lung cancer ; SALLE ; salting-out assisted liquid&ndash ; liquid extraction ; TKI ; tyrosine kinase inhibitor
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 February 2016
- 年：2016
- 卷：1012-1013
- 期：Complete
- 页码：118-123
- 全文大小：528 K
文摘
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The first validated bioanalytical LC–MS/MS assay for afatinib has been reported.
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Salting-out assisted liquid–liquid extraction could be applied for small 10-μl samples.
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The assay has successfully been validated in the 0.5–500 ng/ml range for mouse plasma.
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The assay was successfully used for a pharmacokinetic pilot study in mice.