Evaluation of corncob hemicellulosic hydrolysate for xylitol production by adapted strain of Candida tropicalis

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• 作者：Swati Misra ^{swati.misra11@gmail.com} ; Shailendra Raghuwanshi ; R.K. Saxena ; ^{rksmicrodu@gmail.com}
• 关键词：Xylose ; Corncob hemicellulosic hydrolysate ; C. tropicalis ; Xylitol ; Immobilization
• 刊名：Carbohydrate Polymers
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：92
• 期：2
• 页码：1596-1601
• 全文大小：466 K

文摘

A maximum xylose extraction of 21.98 g/L was obtained in hydrolysate with a solid to liquid ratio of 1:8 (w/v) at 1 % H₂SO₄ and treated for 30 min. The optimized and treated corncob hemicellulosic hydrolysate medium supplemented with (g/L) yeast extract 5.0, KH₂PO₄ 2.0, MgSO₄¡¤7H₂O 0.3 and methanol 10 mL whose pH was adjusted to 4.5 acts as production medium. Under this condition; the adapted strain of C. tropicalis resulted in 1.22-fold increase in xylitol yield and 1.70-fold enhancement in volumetric productivity was obtained as compared to parent strain of C. tropicalis. On concentrating the hydrolysate under vacuum using rotavapor proves to be efficient in terms of improved xylitol yield and productivity over microwave assisted concentration using adapted strain of C. tropicalis. The immobilized cells of C. tropicalis resulted in more than 70 % efficiency up to third cycle. The xylitol production could be scaled up to 10 L fermentor.