An integrated shotgun proteomics and bioinformatics approach for analysis of brain proteins from MCAO model using serial affinity chromatograph with four active ingredients from Shengmai preparations as ligands
Integrated proteomics and bioinformatics approach was established to profile the interaction of components with proteins. MAPK/ERK1/2, CaMKII and VIM played an essential role in the protective effect of SMXZF. It might provide a promising paradigm for target identification and elucidating the mechanism of natural products.