Integrated transcriptomic and proteomic analysis of the bile stress response in probiotic Lactobacillus salivarius LI01
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- 作者：Long-Xian Lv1 ; Ren Yan1 ; Hai-Yan Shi1 ; Ding Shi1 ; Dai-Qiong Fang1 ; Hui-Yong Jiang1 ; Wen-Rui Wu ; Fei-Fei Guo ; Xia-Wei Jiang ; Si-Lan Gu ; Yun-Bo Chen ; Jian Yao ; Lan-Juan Li ; ljli@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：LGG ; Lactobacillus rhamnosus GG ; DGE ; digital gene expression ; iTRAQ ; isobaric tags for relative and absolute quantitation ; RT ; room temperature ; RPKM ; reads per kilobase of exon model per million mapped reads ; DEGs ; differentially expressed genes ; FDR ; false discovery rate ; GO ; Gene Ontology ; CHAPS ; 3-[(3-cholamidopropyl)-dimethyl-ammonio]-1-propane sulfonate ; PMSF ; phenylmethanesulfonyl fluoride ; EDTA ; ethylene diamine tetraacetic acid ; DTT ; dithiothreitol ; IAM ; iodacetamide ; TEAB ; tetraethyla
- 刊名：Journal of Proteomics
- 出版年：2017
- 出版时间：6 January 2017
- 年：2017
- 卷：150
- 期：Complete
- 页码：216-229
- 全文大小：2780 K
文摘
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An integrated transcriptomic and proteomic investigation of bile response mechanism was first performed conducted in L. salivarius by using strain LI01.
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L. salivarius LI01 is a non-BSH-producing strain. Its bile resistance was is mainly based on a highly remodeled cell envelopes and reinforced bile efflux systems.
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Bile-induced alterations in regulatory systems, cell surface, general stress response and central metabolism processes were observed and also to contributed to the bile resistance of LI01.
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Bile-induced potential alterations of LI 01 such as the uptake of aromatic amino acids may be helpful forin liver protection.