Interference-free spectrofluorometric quantification of aristolochic acid I and aristololactam I in five Chinese herbal medicines using chemical derivatization enhancement and second-order calibration methods
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- 作者：Yong Hu ; Hai-Long Wu ; hlwu@hnu.edu.cn ; Xiao-Li Yin ; Hui-Wen Gu ; Rong Xiao ; Li Wang ; Huan Fang ; Ru-Qin Yu
- 关键词：Aristolochic acid ; Aristololactam ; Chinese herbal medicine ; Second-order calibration ; Excitation-emission matrix fluorescence ; Second-order advantage
- 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
- 出版年：2017
- 出版时间：15 March 2017
- 年：2017
- 卷：175
- 期：Complete
- 页码：229-238
- 全文大小：1336 K
- 卷排序：175
文摘
A novel spectrofluorometric method was proposed for the quantification of nephrotoxic AA-I and AL-I in five herbal medicines. A chemical derivatization that converts the non-fluorescent AA-I to high-fluorescent AL-I achieves a high sensitivity. The coupling to second-order calibration methods renders an attractive and green strategy. No significant difference for quantitative results between the proposed strategy and validated LC-MS/MS method.