A rapid triple-mode fluorescence switch assay for immunoglobulin detection by using quantum dots-gold nanoparticles nanocomposites
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- 作者：Qi WangAuthor Vitae ; Xuan FuAuthor Vitae ; Xi HuangAuthor Vitae ; Fangyi WuAuthor Vitae ; Meihu MaAuthor Vitae ; Zhaoxia Cai ; caizhaoxia@mail.hzau.edu.cn" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae
- 关键词：Immunoglobulin ; IgY ; Triple-mode nanosensor ; Fluorescence resonance energy transfer ; Anti-IgY ; Staphylococcal protein A
- 刊名：Sensors & Actuators: B. Chemical
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：779-786
- 全文大小：1546 K
文摘
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We report a triple-modenano sensorbased on CdTe QDs and AuNPs for discriminative detection of immunoglobulin.
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The immune interaction between QDs-SPA and anti-IgY-AuNPs leads to donorquenching.
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Anti-IgY-AuNPs peel off fromQDs-SPA and bind to IgY, resulting in QDs fluorescence restoration.
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Fluorescence intensity increased linearly with increasing IgY concentrations from 5 to 200 ng/mL with LOD of 1.16 ng/mL.