Inhibitory effect of gold nanoparticles conjugated with interferon gamma and methionine on breast cancer cell line

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• 作者：Nastaran Mohseni¹ ; Fatemeh Salehi Sarvestani² ; Mehdi Shafiee Ardestani³ ; Fatemeh Kazemi-Lomedasht¹ ; Masoud Ghorbani⁴ ; ⁵ ; ^{Mghorbani2000@yahoo.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Gold nanorods ; Breast cancer cell line ; Interferon gamma ; Near-infrared laser
• 刊名：Asian Pacific Journal of Tropical Biomedicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：6
• 期：2
• 页码：173-178
• 全文大小：1076 K

文摘

To develop a gold nanoparticles complex conjugated with interferon-gamma (IFN-γ) and methionine along with application of hyperthermia using near-infrared laser beams for the treatment of cancer cells.

Methods

Gold nanorods (10 nm) were conjugated with IFN-γ and methionine using carbodiimide family and characterized after purification by dialysis bags. Breast cancer cells were cultured and incubated with gold nanorods at different concentrations followed by irradiation with near-infrared laser beam. Samples were then evaluated for their viability in order to determine the effect of treatment and variables by MTT assy.

Results

Zetasizer results confirmed the conjugation of gold nanorods with methionine and IFN-γ. The median percentage of cell viability in 0.30 μg/mL concentration of gold nanorods was 82%. The cell viability reached to 85% at the same concentration of gold nanorods, which existed in the assayed complex. The results of MTT assay showed that the 0.60 μg/mL concentration of gold nanoparticles complex was toxic on tumor cells (P < 0.05). After exposure to hyperthermia, the viability of cells at 6 min decreased to 77% in 0.30 μg/mL concentration of gold nanorods complex.

Conclusions

The size and concentration of gold nanorods was not cytotoxic. However, their presence during irradiation near-infrared laser increased the number of dead cells during the treatment of cells.