Magnetic fluid — a novel approach to treat amyloid-related diseases

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• 作者：Andrea Antosova ; Katarina Siposova ; Martina Koneracka ; Vlasta Zavisova ; Zuzana Daxnerova ; Ivo Vavra ; Diana Fedunova ; Jaroslava Bagelova ; Peter Kopcansky ; Zuzana Gazova
• 关键词：Protein aggregation ; Amyloid fibril ; Magnetic fluid ; Protein ; Nanomedicine
• 刊名：Physics Procedia
• 出版年：2010
• 出版时间：2010
• 年：2010
• 卷：9
• 期：Complete
• 页码：262-265
• 全文大小：383 K

文摘

Protein amyloid aggregates are associated with several human pathologies termed amyloid-related diseases. We have investigated effect of two magnetic fluids (MFs) — electrostatically stabilized Fe₃O₄ magnetic nanoparticles (MF1) and sterically stabilized Fe₃O₄ magnetic nanoparticles by sodium oleate with adsorbed BSA (MF2) on amyloid aggregation of two proteins - human insulin and hen egg lysozyme. We have found that both MF1 and MF2 are able to interact with amyloid fibrils in vitro resulting into decreasing of amyloid aggregates. The extent of fibril disruption depends on MF concentration with extensive reduction of amyloid aggregates, 90 % for lysozyme and 70 % for insulin (ratio protein: MF=1:5). The obtained results suggest that magnetite component of MF play significant role in the process of amyloid fibril depolymerisation. Our findings indicate that MF1 and MF2 have potential to be used for treatment of amyloid diseases.