Development and method validation for determination of 128 pesticides in bananas by modified QuEChERS and UHPLC-MS/MS analysis

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• 作者：Raphaella P. Carneiro^a ; Fabiano A.S. Oliveira^a ; Fernando D. Madureira^a ; ^{fernando.madureira@agricultura.gov.br} ; Gilsara Silva^a ; Wesley R. de Souza^a ; Renata Pereira Lopes^b ; ^{renatapereiralopes@yahoo.com.br} ; ^{renatapl@ufmg.br}
• 关键词：Pesticides ; Banana ; QuEChERS ; UHPLC&ndash ; MS/MS
• 刊名：Food Control
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：33
• 期：2
• 页码：413-423
• 全文大小：722 K

文摘

A multiresidue method for the quantification of 128 pesticides in banana is described. It involves the application of a modified QuEChERS procedure followed by UHPLC-MS/MS (Ultra High Performance Liquid Chromatography coupled to Tandem Mass Spectrometry) analysis. The method was validated according to the European Union SANCO/12495/2011 guidelines and Brazilian Manual of Analytical Quality Assurance. The validation levels were 10.0; 25.0; 50.0 and 100?¦Ìg?kg^?1. Acceptable values were?obtained for the following parameters: linearity, limit of detection - LOD (5.00?¦Ìg?kg^?1) and?limit?of quantification - LOQ (10.0?¦Ìg?kg^?1), except for fenamiphos and mevinphos (LOD?=?7.5?¦Ìg?kg^?1 and?LOQ?=?25?¦Ìg?kg^?1), trueness (for the levels: 10.0, 25.0, 50.0 and 100?¦Ìg?kg^?1 the recovery assays values were between 70 and 120 % ) except for methamidophos at 10?¦Ìg?kg^?1 level (67.5 % ), intermediate precision (<20.0 % ) and measurement uncertainty tests (<50.0 % ). These results demonstrate the applicability of this method in the routine practice by the laboratories of Ministry of Agriculture, Livestock and Food Supply of Brazil that attend the National Control Plan for Residues and Contaminants (PNCRC).