Acetylated starch nanocrystals: Preparation and antitumor drug delivery study

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• 作者：Huaxi Xiao^a ; Tao Yang^a ; Qinlu Lin^a ; ^{linedu2018@163.com" class="auth_mail" title="E-mail the corresponding author} ; Gao-Qiang Liu^a ; ^{gaoliuedu@163.com" class="auth_mail" title="E-mail the corresponding author} ; ^{gaoliuedu@csuft.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Lin Zhang^a ; Fengxiang Yu^b ; Yuejiao Chen^a
• 关键词：Broken rice ; Acetylated starch nanoparticles ; Drug delivery
• 刊名：International Journal of Biological Macromolecules
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：89
• 期：Complete
• 页码：456-464
• 全文大小：2296 K

文摘

In this study, we developed a new nanoparticulate system for acetylated starch nanocrystals (ASN) using broken rice. ASN with different degrees of substitution (DS) of 0.04, 0.08 and 0.14 were prepared using acetic anhydride as acetylating agent through reaction with starch nanocrystals (SN). The resulting ASN were investigated for the capability to load and release doxorubicin hydrochloride (DOX), and the antitumor activities of DOX-loaded SN and DOX-loaded ASN were evaluated as potential drug delivery systems for cancer therapy. Cellular uptake and cytotoxicity of nanocrystals and the DOX-loaded nanocrystals were investigated using fluorescence microscopy and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Compared with acetylated starches (AS) and native starches (NS), ASN with DS 0.14 loaded up to 6.07% of DOX with a higher loading efficiency of 91.1% and had steadier drug-release rates. Toxicity analysis using the rat hepatocytes model suggested that ASN was biocompatible and could be used for drug delivery. Furthermore, ASN were taken up by cancer cells in vitro and significantly enhanced the cytotoxicity of DOX against HeLa human cervical carcinoma cells. The IC50 value of DOX-loaded ASN-DS 0.14 was 3.8 μg/mL for 24 h of treatment, which was significantly lower than that of free DOX (21 μg/mL). These results indicate that the prepared ASN using broken rice is a promising vehicle for the controlled delivery of DOX for cancer therapy.