Genomic characterization and expression analysis of four apolipoprotein A-IV paralogs in Senegalese sole (Solea senegalensis Kaup)

详细信息    查看全文

• 作者：J. Roman-Padilla^a ; A. Rodrí ; guez-Rua^a ; M.G. Claros^b ; I. Hachero-Cruzado^a ; M. Manchado^a ; ^{manuel.manchado@juntadeandalucia.es" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AI ; anterior intestine ; B ; brain ; BCIP ; bromo-chloro-indolyl phosphate ; CM ; chylomicrons ; DEPC ; diethylpyrocarbonate ; dpf ; days post fertilization ; dph ; days post hatch ; H ; heart ; hpf ; hours post fertilization ; HybMix ; hybridization mix ; HDL ; high-density lipoproteins ; kb ; kilobases ; LDL ; low-density lipoproteins ; LV ; liver ; MAB ; Malic acid buffer ; MeOH ; methanol ; OG ; oil globule ; OV ; otic vesicle ; ORF ; open reading frame ; ML ; maximum likelihood ; PBT ; 1  ; × ;   ; PBS  ; +  ; 0.1% Twe
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：191
• 期：Complete
• 页码：84-98
• 全文大小：3995 K

文摘

The apolipoprotein A-IV (ApoA-IV) plays a key role in lipid transport and feed intake regulation. In this work, four cDNA sequences encoding ApoA-IV paralogs were identified. Sequence analysis revealed conserved structural features including the common 33-codon block and nine repeated motifs. Gene structure analysis identified four exons and three introns except for apoA-IVAa1 (with only 3 exons). Synteny analysis showed that the four paralogs were structured into two clusters (cluster A containing apoA-IVAa1 and apoA-IVAa2 and cluster B with apoA-IVBa3 and apoA-IVBa4) linked to an apolipoprotein E. Phylogenetic analysis clearly separated the paralogs according to their cluster organization as well as revealed four subclades highly conserved in Acanthopterygii. Whole-mount analyses (WISH) in early larvae (0 and 1 day post-hatch (dph)) showed that the four paralogs were mainly expressed in yolk syncytial layer surrounding the oil globules. Later, at 3 and 5 dph, the four paralogs were mainly expressed in liver and intestine although with differences in their relative abundance and temporal expression patterns. Diet supply triggered the intensity of WISH signals in the intestine of the four paralogs. Quantification of mRNA abundance by qPCR using whole larvae only detected the induction by diet at 5 dph. Moreover, transcript levels increased progressively with age except for apoA-IVAa2, which appeared as a low-expressed isoform. Expression analysis in juvenile tissues confirmed that the four paralogs were mainly expressed in liver and intestine and secondary in other tissues. The role of these ApoA-IV genes in lipid transport and the possible role of apoA-IVAa2 as a regulatory form are discussed.