In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes

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• 作者：Miriam P. Cervantes^a ; J. Manuel Palomino^a ; Muhammad Anzar^b ; Reuben J. Mapletoft^c ; Gabriela F. Mastromonaco^d ; Gregg P. Adams^a ; ^{gregg.adams@usask.ca}
• 关键词：Blastocyst ; Oocyte ; In vitro fertilization ; In vitro ; In vivo maturation
• 刊名：Theriogenology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：89
• 期：Complete
• 页码：122-130
• 全文大小：951 K
• 卷排序：89

文摘

Experiments were conducted in wood bison to determine the effect of additional maturation time on embryo development of in vivo matured oocytes. In experiment 1, cumulus-oocyte complexes (COC) were collected 30 hours after hCG treatment in superstimulated wood bison, and expanded COC were fertilized immediately or after 4 hours of additional in vitro maturation. Embryo development was assessed on Days 3, 7, and 8 (Day 0 = day of fertilization). No difference in cleavage rate was detected (55.3% vs. 60.5%, P = 0.82), but the Day 8 blastocyst rate was higher after an additional 4 hours of in vitro maturation time (44.7 vs. 18.4%, P = 0.03). In experiment 2, COC were collected at either 30 hours or 34 hours after hCG treatment. Expanded COC from the 30 hours group were fertilized after 4 hours of in vitro maturation, whereas those from the 34 hours group were fertilized immediately. A higher cleavage rate (74.3 vs. 57.0%) and blastocyst rate (54.1 vs. 37.2%) were found in the 34 hours group versus the 30 hours group (P < 0.05). In conclusion, an additional short period of in vitro maturation, or an extended period of in vivo maturation are beneficial for in vitro embryo production in wood bison.