Three lipases were applied to hydrolyze the floatable grease in the food waste. Animal fat and vegetable oil are applied as substrates for anaerobic digestion. Three lipids are hydrolyzed in the conditions of 24 h, 1000–1500 μL and 40–50 °C. The digestion time was shortened by 10–40 d for three lipids treated by lipases. The biomethane production rate of the hydrolysis products was enhanced 26.9–157.7%.