Analysis of Calcifications in Patients with Coral Reef Aorta

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• 作者：Georg Schlieper ; Dirk Grotemeyer ; Anke Aretz ; Leon J. Schurgers ; Thilo Krü ; ger ; Hermann Rehbein ; Thomas E. Weirich ; Ralf Westenfeld ; Vincent M. Br ; enburg ; Frank Eitner ; Joachim Mayer ; Jü ; rgen Floege ; Wilhelm S ; mann ; Markus Ketteler
• 刊名：Annals of Vascular Surgery
• 出版年：2010
• 出版时间：April 2010
• 年：2010
• 卷：24
• 期：3
• 页码：408-414
• 全文大小：1616 K

文摘

Background

Coral reef aorta is a rare vascular disease with intraluminal calcifications of the dorsal part of the visceral aorta. The pathogenesis of this disease with its topographic and morphologic characteristics is unknown. The aim of our study was to investigate calcification inhibitors and the ultrastructure of calcifications in patients with coral reef aorta.

Methods

Ten patients with coral reef aorta were examined. Calcified specimens were investigated by immunohistochemical techniques for the expression of the calcification inhibitors matrix gla protein (MGP) and fetuin-A. Vessel walls were also assessed by electron microscopic techniques including electron energy-lost spectroscopy, electron dispersive spectroscopy, and electron diffraction. Sera of patients were analyzed for fetuin-A, uncarboxylated MGP (ucMGP), and osteoprotegerin.

Results

As assessed by immunohistochemistry, most MGP was detected in the vicinity of calcified regions. Serum levels of the calcification inhibitors ucMGP, fetuin-A, and osteoprotegerin were 370 ± 107 nmol/L, 0.57 ± 0.03 g/L, and 5.64 ± 0.79 pmol/L, respectively. Ultrastructural analysis of calcified specimens showed a core-shell structure with multiple calcification nuclei. Calcifications displayed a fine-crystalline character, and elemental analysis revealed hydroxyl apatite as the chemical compound.

Conclusion

The coral reef aorta represents an extreme exophytic growth of vascular calcification with multiple nuclei which resemble typical media calcification. Positive vascular immunostaining and low serum levels of both fetuin-A and ucMGP suggest a pathophysiologic role of these calcification inhibitors in the development of coral reef aorta.