Imaging In focus: Reflected light imaging: Techniques and applications

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• 作者：Emily J. Guggenheim^a ; ^b ; Iseult Lynch^b ; Joshua Z. Rappoport^c ; ^{Joshua.rappoport@northwestern.edu}
• 关键词：CLSM ; Confocal Laser Scanning Microscopy ; DF ; Darkfield ; FCM ; Fluorescence Confocal Microscopy ; ICPMS ; Inductively Coupled Plasma Mass Spectrometry ; IRM ; Interference Reflectance Microscopy ; NIR ; Near-Infrared ; NP ; Nanoparticles ; OCT ; Optical Coherence Tomography ; RCM ; Reflectance Confocal Microscopy ; RI ; Refractive index ; SNR ; Signal to Noise Ratio ; SIM ; Structured Illumination Microscopy ; TIRM ; Total Internal Reflectance Microscopy
• 刊名：The International Journal of Biochemistry & Cell Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：83
• 期：Complete
• 页码：65-70
• 全文大小：733 K
• 卷排序：83

文摘

Reflectance imaging is a broad term that describes the formation of images by the detection of illumination light that is back-scattered from reflective features within a sample. Reflectance imaging can be performed in a variety of different configurations, such as confocal, oblique angle illumination, structured illumination, interferometry and total internal reflectance, permitting a plethora of biomedical applications. Reflectance imaging has proven indispensable for critical investigations into the safety and understanding of biomedically and environmentally relevant nano-materials, an area of high priority and investment. The non-destructive in vivo imaging ability of reflectance techniques permits alternative diagnostic strategies that may eventually facilitate the eradication of some invasive biopsy procedures. Reflectance can also provide additional structural information and clarity necessary in fluorescent based in vivo studies. Near-coverslip interrogation techniques, such as reflectance interferometry and total internal reflection, have provided a label free means to investigate cell-surface contacts, cell motility and vesicle trafficking in vivo and in vitro. Other key advances include the ability to acquire superresolution reflectance images providing increased spatial resolution.