S - and N-alkylating agents diminish the fluorescence of fluorescent dye-stained DNA

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• 作者：Robert Giesche^a ; Harald John^a ; Kai Kehe^b ; ^c ; Annette Schmidt^a ; Tanja Popp^a ; ^b ; Frank Balzuweit^e ; ^d ; Horst Thiermann^a ; Thomas Gudermann^b ; ^e ; Dirk Steinritz^a ; ^b ; ^{dirksteinritz@bundeswehr.org}
• 关键词：Chemical warfare agents ; DNA staining ; Sulfur mustard ; Fluorescent dyes ; Mass spectrometry ; Ethidium bromide
• 刊名：Chemico-Biological Interactions
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：262
• 期：Complete
• 页码：12-18
• 全文大小：873 K
• 卷排序：262

文摘

DNA was exposed to sulfur mustard (SM) and stained with fluorescence dyes (FD). Fluorescence decreased in the presence of SM and related alkylating compounds. Interactions between FD and SM or DNA-crosslinks were ruled out as cause. Protons caused the loss of fluorescence which was prevented by appropriate buffers. DNA-quantification using FD in presence of SM is possible considering our results.