An aptamer-based effective method for highly sensitive detection of chloramphenicol residues in animal-sourced food using real-time fluorescent quantitative PCR

详细信息    查看全文

• 作者：Ye Duan^a ; ¹ ; Lihui Wang^a ; ¹ ; Zhiqiang Gao^b ; Huishan Wang^c ; Hexiao Zhang^b ; ^{zhanghx@bjciq.gov.cn} ; Hao Li^a ; ^{lihao@mail.buct.edu.cn} ; ^{lihaoh@163.com}
• 关键词：B&W ; washing and binding ; CAP ; chloramphenicol ; Ct ; cycle threshold ; dsDNA ; double stranded DNA ; ELISA ; enzyme linked immunosorbent assay ; EU ; European Union ; FF ; florfenicol ; FRET ; fluorescence resonance energy transfer ; GC ; gas chromatography ; LC ; liquid chromatography ; LC-MS ; liquid chromatography mass spectrometry ; LOD ; limit of detection ; MRPL ; minimum required performance limit ; qRT-PCR ; real-time fluorescent quantitative PCR ; SELEX ; systematic evolution of ligands by exponential enrichmen
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 April 2017
• 年：2017
• 卷：165
• 期：Complete
• 页码：671-676
• 全文大小：608 K
• 卷排序：165

文摘

A novel aptamer-based system for CAP detection was established. The detection limit of this aptamer-based system was 0.1 ng/mL. The aptamer-based system showed high selectivity against CAP's structure analogs. The recovery rate of CAP from spiked milk samples ranged from 94.0% to 102.0%.