Antidepressant-like effects of albiflorin extracted from Radix paeoniae Alba

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• 作者：Yu-Lu Wang^a ; ^b ; Jing-Xia Wang^c ; Xiao-Xu Hu^b ; Li Chen^b ; Zhi-Kun Qiu^d ; Nan Zhao^b ; Zi-Dan Yu^b ; Shu-Zheng Sun^b ; Yuan-Yuan Xu^b ; Yan Guo^b ; Chang Liu^b ; You-Zhi Zhang^b ; Yun-Feng Li^b ; ^{lyf619@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Chang-Xi Yu^a ; ^{changxiyu@mail.fjmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Albiflorin (PubChem CID ; 51346141) ; Paeoniflorin (PubChem CID ; 75124163) ; Fluoxetine hydrochloride (PubChem CID ; 62857) ; Dopamine (PubChem CID ; 681) ; Norepinephrine (PubChem CID ; 439260) ; Adrenalin (PubChem CID ; 5816) ; 3 ; 4-Dihydroxyphenylacetic acid (PubChem CID ; 547) ; Homovanillic acid (PubChem CID ; 1738) ; 5-Hydroxytryptamine (PubChem CID ; 5202) ; 5-Hydroxyindoleacetic acid (PubChem CID ; 1826)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：17 February 2016
• 年：2016
• 卷：179
• 期：Complete
• 页码：9-15
• 全文大小：1292 K

文摘

Albiflorin, a monoterpene glycoside, is a main component of Radix paeoniae Alba, which could be a Chinese herbal medicine used in the treatment of psychiatric disorders. However, the exact role of albiflorin in depression is poorly understood.

Aim of the study

The current study aimed to evaluate the antidepressant effect of albiflorin in mice and rats, and the possible mechanism was also determined.

Materials and methods

The antidepressant-like effects of albiflorin was determined by using animal models of depression including forced swim and tail suspension tests in mice and chronic unpredictable stress (CUS) in rats. The acting mechanism was explored by determining the effect of albiflorin on the expression of brain-derived neurotrophic factor (BDNF) in the hippocampus by western blot and the levels of monoamine in the hippocampus by HPLC.

Results

Our results showed that 7 days treatment with albiflorin significantly decreased immobility time in the forced swimming test (FST) and the tail suspension test (TST) at doses of 3.5, 7.0 and 14.0 mg/kg without alter the locomotor activity in mice. Moreover, western blot analysis showed that albiflorin could increase the expression of BDNF in the hippocampus. We further exposed rats to a chronic unpredictable stress (CUS) protocol for a period of 35 d to induce depressive-like behaviors. We found that chronic treatment with albiflorin, at doses of 7.0 and 14.0 mg (i.g., once daily for 35 d), restored the sucrose preference in CUS rats. In the open-field test, albiflorin significantly increased the number of crossings and rearings in the CUS rats at three doses. Moreover, chronic treatment with albiflorin up-regulated the hippocampal BDNF expression levels and the hippocampal 5-HT, 5-HIAA, and NA levels.

Conclusion

Albiflorin produced significant antidepressant-like effects, which were closely related to the hippocampal 5-HT/NE increase and BDNF expression. Our data indicated that albiflorin could be a potential anti-depressant drug.