The Type 2 dUTPase of Bacteriophage ϕNM1 Initiates Mobilization of Staphylococcus aureus Bovine Pathogenicity Island 1
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- 作者：Rosanne L.L. Hill ; Terje Dokland ; dokland@uab.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：SaPI ; Staphylococcus aureus pathogenicity island ; CFU ; colony-forming units ; BACTH ; bacterial two-hybrid ; EM ; electron microscopy ; PFU ; plaque-forming units
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：16 January 2016
- 年：2016
- 卷：428
- 期：1
- 页码：142-152
- 全文大小：985 K
文摘
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SaPIs are mobilized through derepression by phage proteins.
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SaPIbov1 is derepressed by phage ϕNM1 Dut, a type 2 α-helical dUTPase (DutNM1).
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DutNM1 interacts with the SaPIbov1 Stl repressor, releasing it from its DNA target.
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Interaction with Stl leads to inhibition of the dUTPase activity of DutNM1.
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This work suggests a novel mechanism for the derepression that leads to SaPI mobilization.