Molecular studies on capsular polysaccharide biosynthesis

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• 作者：Donatella Cimini ; Ambra Viggiani ; Odile Francesca Restaino ; Andrea Panariello ; Mario De Rosa ; Chiara Schiraldi
• 关键词：Biosorption ; Rhizopus arrhizus ; Rice bran ; Industrial wastewater ; Nickel removal
• 刊名：Journal of Biotechnology
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：131
• 期：2, Supplement 1
• 页码：S203
• 全文大小：55 K

文摘

Trichokonins are peptaibols produced by Trichoderma koningii SMF2. The main isoforms are Trichokonin VI, Trichokonin VII and Trichokonin VIII. The solid-state fermentation (SSF) was applied for the production of Trichokonin VI. The fermentation factors, which included inoculum size, incubation temperature, initial moisture content and initial pH, were investigated and optimized by response surface methodology. The maximum Trichokonin VI production (4.07 mg/g dry substrate) was achieved by employing inoculum size of 18 % , incubation temperature at 24.3 °C, initial moisture content of 77.5 % and initial pH at 5.0. Furthermore, gel filtration and preparative HPLC were used for separation of Trichokonin VI from a crude extract of the T. koningii SMF2 culture. With this preparative purification protocol under optimized fermentation conditions, 146.20 mg Trichokonin VI was obtained from 1 kg solid cultures. It has been shown that the obtained Trichokonin VI is more than 95 % in purity. This is the first report on optimization of peptaibols production in SSF with high content. An efficient method for the preparative purification of Trichokonin VI is also proposed.