Urinary epidermal growth factor, monocyte chemotactic protein-1, and β2-microglobulin in children with ureteropelvic junction obstruction

详细信息    查看全文

• 作者：Fabio Bartoli^a ; ^{bartolifabio@libero.it} ; Rosa Penza^b ; Gabriella Aceto^b ; Francesco Niglio^a ; Olinda D'Addato^b ; Valentina Pastore^a ; Vittoria Campanella^a ; Simona Magaldi^c ; Carla Lasalandra^a ; Giovanna Di Bitonto^b ; Loreto Gesualdo^c
• 关键词：Hydronephrosis ; EGF ; MCP-1 ; β ; 2-Microglobulin ; Children ; Ureteropelvic junction obstruction
• 刊名：Journal of Pediatric Surgery
• 出版年：2011
• 出版时间：March 2011
• 年：2011
• 卷：46
• 期：3
• 页码：530-536
• 全文大小：148 K

文摘

Background/Purpose

We demonstrated down-regulation of epidermal growth factor (EGF) and up-regulation of monocyte chemotactic protein-1 (MCP-1) in the renal parenchyma in children who underwent pyeloplasty for ureteropelvic junction obstruction (UPJO). These findings were paralleled by urinary levels of EGF and MCP-1 before and after surgery. The aim of this study is to evaluate the urinary excretion of these cytokines and β2-microglobulin (β2M) in children with urine flow impairment at the ureteropelvic junction or who underwent pyeloplasty.

Methods

Seventy-six patients with UPJO and 30 normal children (CTRL) were enrolled in the study. The UPJO patients were divided into obstructive (12), functional (36), and operated (28). Epidermal growth factor, MCP-1, and β2M urinary levels were measured by enzyme-linked immunosorbent assay and normalized to urine creatinine.

Results

Urinary β2M and MCP-1 increased significantly in the UPJO groups compared with the CTRL and significantly improved in the operated group. The obstructive group displayed reduced EGF excretion compared with the CTRL group. The urinary (u)EGF/uMCP-1, and uEGF/uβ2M ratios significantly decreased in both untreated groups. In the operated group, these ratios improved significantly.

Conclusions

The present study substantiates the role of urinary EGF, MCP-1, and β2M as markers of tubulointerstitial damage in human obstructive nephropathy. Furthermore, it suggests that surgical intervention is effective in the management of children with UPJO.