Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain

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• 作者：Á ; urea Regina Telles Pupulin¹ ; ⁵ ; Lí ; via Bracht² ; Má ; rcia Machado de Oliveira Dalalio³ ; Francielli Maria de Souza Silva-Comar⁴ ; Bruno Ambró ; sio da Rocha⁴ ; Franciele Queiroz Ames⁴ ; Roberto Kenji Nakamura Cuman⁴ ; Ciomar Aparecida Bersani-Amado⁴ ; ^{ciomar.amado@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Trypanosoma cruzi ; Canova medication ; Cytokines
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：9
• 期：9
• 页码：860-865
• 全文大小：399 K

文摘

To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.

bsSec_2">Methods

bspara0015">Animals were divided into five groups: non-treated infected animals (I); benznidazole-treated infected animals (Bz; 100 mg/kg body weight, single daily dose by gavage); Canova medication (CM) treated infected animals (CM; 0.2 mL/animal, single daily dose by gavage); benznidazole- and Canova medication-treated infected animals with the above-mentioned dose (Bz+CM); and non-infected animals (C). TNF-α and IL-10 levels were determined in serum aliquots after 4, 7, 10, 13, and 29 days of infection. An ELISA technique was employed with R&D System Inc. antibody pairs.

bsSec_3">Results

bspara0020">A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10th day after infection, coupled with a IL-10 decrease on the 13th day after infection when compared with the other experimental groups.

bsSec_4">Conclusions

bspara0025">CM may change the balance between plasma cytokine levels (TNF-α and IL-10) in mice infected with Y strain T. cruzi, with important consequences leading towards a more severe infection.