Inhibitory effects of 伪-cyperone on adherence and invasion of avian pathogenic Escherichia coli O78 to chicken type II pneumocytes
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- 作者：Li-Yan Zhang ; Shuang Lv ; Shuai-Cheng Wu ; Xun Guo ; Fang Xia ; Xi-Rou Hu ; Zhou Song ; Cui Zhang ; Qian-Qian Qin ; Ben-Dong Fu ; fubendong96@163.com" class="auth_mail ; fubd@jlu.edu.cn" class="auth_mail ; Peng-Fei Yi ; Hai-Qing Shen ; Xu-Bin Wei
- 关键词：APEC-O78 ; Chicken type II pneumocytes ; Adherence ; Invasion ; Actin cytoskeleton polymerization ; 伪-cyperone
- 刊名：Veterinary Immunology and Immunopathology
- 出版年：15 May, 2014
- 年：2014
- 卷：159
- 期：1-2
- 页码：50-57
- 全文大小：1183 K
文摘
Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood-gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood-gas barrier. However, the mechanism of APEC injuring type II pneumocytes remains unclear. 伪-cyperone can inhibit lung cell injury induced by Staphylococcus aureus. In order to explore whether 伪-cyperone regulates the adherence and invasion of APEC-O78 to chicken type II pneumocytes, we successfully cultured chicken type II pneumocytes. The results showed that 伪-cyperone significantly decreased the adherence of APEC-O78 to chicken type II pneumocytes. In addition, 伪-cyperone inhibited actin cytoskeleton polymerization induced by APEC-O78 through down regulating the expression of Nck-2, Cdc42 and Rac1. These results provide new evidence for the prevention of colibacillosis in chicken.