Fucosyltransferases produce N-glycans containing core l-galactose

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• 作者：Hiroyuki Ohashi^a ; Takao Ohashi^a ; Hiroyuki Kajiura^a ; Ryo Misaki^a ; Shinichi Kitamura^b ; Kazuhito Fujiyama^a ; ^{fujiyama@icb.osaka-u.ac.jp}
• 关键词：Arabidopsis ; Fucosyltransferase ; GDP-l-galactose ; GDP-d-mannose 3&prime ; 5&prime ; -epimerase ; N-glycan
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：29 January 2017
• 年：2017
• 卷：483
• 期：1
• 页码：658-663
• 全文大小：823 K
• 卷排序：483

文摘

GDP-l-galactose was prepared in preparative scale using GDP-d-mannose-3′,5′-epimerase (AtGME) and recycle-HPLC. Mouse α1,6-fucosyltransferase (MmFUT8) transferred l-galactose to N-glycan. Arabidopsis α1,3-fucosyltransferase (AtFucTA) transferred l-galactose to N-glycan.