Preparation of l-phenylalanine imprinted polymer based on monodisperse hybrid silica microsphere and its application on chiral separation of phenylalanine racemates as HPLC stationary phase

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• 作者：Zhaohui Zhang^a ; ^b ; ^c ; ^{zhaohuizhang77@163.com} ; Minglei Zhang^a ; Yunan Liu^a ; Xiao Yang^a ; Lijuan Luo^a ; Shouzhuo Yao^b
• 关键词：Phenylalanine racemates ; Molecularly imprinted polymer ; Chiral separation ; ¦Â-Cyclodextrin
• 刊名：Separation and Purification Technology
• 出版年：2012
• 出版时间：5 March, 2012
• 年：2012
• 卷：87
• 期：Complete
• 页码：142-148
• 全文大小：643 K

文摘

In this paper, a l-phenylalanine (l-Phe) imprinted polymer (MIP) based on monodisperse hybrid silica microspheres (MH-SiO₂) with CHCH₂ groups was synthesized by surface imprinting technique. The MH-SiO₂ was synthesized by a sol-gel process in aqueous media using tetraethylorthosilicate (TEOS) and 3-methylacryloxypropyl trimethoxysilane (MATES) as the precursors. The morphologies of MH-SiO₂ and MIP were characterized with scanning electron microscope (SEM) and the imprinted shell thickness on the surface of MH-SiO₂ was calculated as 0.5 ¦Ìm. Compared with the imprinted polymer prepared only by ¦Â-cyclodextrin (¦Â-CD) or methacrylic acid (MAA) as functional monomer, the imprinted polymer prepared with both ¦Â-CD and MAA as binary functional monomers hold the highest adsorption capacity. Under the optimum chromatographic conditions, a complete baseline separation of phenylalanine racemates was observed only using the column packed with the imprinted polymer prepared with both ¦Â-CD and MAA as binary functional monomers. And the separation factor and resolution of the imprinted polymer towards phenylalanine racemates were calculated as 1.41 and 1.46, respectively, which is higher than that of other imprinted polymers.