Preparation for aflatoxin B₁-cationized bovine serum albumin based on Mannich-type reaction

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• 作者：Youxiang Zhou ; Jiajia Wu ; Wei Yu ; Ying Xu ; Ping Wang ; Bijun Xie ; Fusheng Chen
• 关键词：Aflatoxin B₁ ; Cationized protein ; Mannich-type reaction
• 刊名：Journal of Immunological Methods
• 出版年：2007
• 出版时间：1 December 2007
• 年：2007
• 卷：328
• 期：1-2
• 页码：79-88
• 全文大小：538 K

文摘

Aflatoxin B₁ (AFB₁), which is commonly found in agricultural commodities, is one of the most potent carcinogenic mycotoxins. To ensure food safety, rapid and low-cost immunological methods have been applied to detect AFB₁ worldwide. A key step in these immunological methods is coupling AFB₁ to carrier proteins; AFB₁ is usually deviated to AFB₁-oxime and coupled to carrier proteins to form the AFB₁-oxime–protein conjugate. In the current research, AFB₁ was directly coupled with cationized bovine serum albumin (cBSA) using a method based on Mannich-type principles. The coupling effects were investigated with different initial molar ratios of AFB₁ to cBSA. The conjugate molar ratio was 6.4:1 when the initial molar ratio was 40:1. The cationized proteins and their conjugates were identified by UV–Vis and FT-IR spectra, which showed the characteristic bands of the ethylendiamine group and AFB₁, respectively. After BALB/c mice were immunized with AFB₁–cBSA, a quicker immunological response and a similar sensitivity of antisera against AFB₁ were observed, compared with immunization by AFB₁-oxime–BSA. This suggests that the Mannich-type reaction might be an alternative method of preparation for AFB₁–protein.